Anatomical: immunohistochemistry and retrograde/anterograde tract tracing.

Immunohistochemistry can be used to visualize the location of proteins of interest in neural tissue.

A combination of retrograde tracing techniques and immunohistochemistry reveals which neurons within the ventral tegmental area are activated (fos) by a given treatment. In Panel B, dopaminergic neurons are identified (TH). In Panel C, prefrontal cortex-projecting neurons are identified (FG). Double-labeled cells are indicated by the *.

back to Methods

Department of Psychology
1202 West Johnson Street
University of Wisconsin at Madison
Madison, WI 53706-1696

© 2001 by the Board of Regents of the University of Wisconsin System.
Last Revised:
comments